Introduction

Does your research require antibodies against newly discovered or poorly characterized proteins?  If so, we would be glad to recommend AccuMono, our custom monoclonal antibody service.  This program was developed with three goals in mind: to provide you antibodies against any target of your specification; to maximize your chance of success with any application(s) of your choice; and to lower your cost.

Package Options Based on Antigen Types

Scientists consider the selection of antigens a key determinant on the outcome of an antibody project.  However, no consensus exists as to which type of antigens would yield the best results.  The truth is, a good choice of antigen(s) always depends on the nature of your protein and the nature of your application (see Table 1).  For this reason, our laboratories work with all three major types of antigens: peptides, soluble protein fragments and full-length recombinant proteins (long soluble fragments or full-length proteins are usually reserved for very "difficult" proteins).  With AccuMono, you could start a project with any antigen type, a combination of types and multiple antigens.

Table 1.  Available Antigen Options for Custom Monoclonal Antibody Service.  Abbreviation for antibody applications: WB, Western blot; IF, immunofluorescence; IHC, immunohistochemistry; ICC, immunocytochemistry; IP, immunoprecipitation; ChIP, chromatin immunoprecipitation; FC, flow cytometry.  *For full-length protein antigens, we suggest that customer supply cDNA for cloning and expression.

Procedure

Customer could start a project simply by submitting a protein sequence (for peptide or fragment antigen).  Our technical staff would then analyze the sequence with the proprietary AccuTope algorithm.  The software identifies 2 types of potential antigens from the unique and exposed portions of the protein: those that lack secondary and tertiary structures; and those that fold autonomously.  Such antigens (called "AccuTopes antigens") would appear the same in the peptide or fragment form or in the context of the native protein.  As a result, antibodies raised against them would likely recognize the native protein.

One or several antibodies are then used to immunize mice by our modified protocols.  Fusion and clone selection are carried out with our advanced hybridoma techniques.  Finally, our strict screening methods would ensure that only the best performing antibodies are delivered to customer.

Within 120 days of project initiation, we would send customer lyophilized ascites fluid (~80% antibody) of each successful clone for testing.  Based on customer feedback, purified antibodies and cell lines of working clones will be supplied in 3-4 weeks.

Advantages

The discovery of new, performing antibodies has remained a time-consuming and costly process.  The AccuMono program differs from the traditional approach in several important aspects: first, the tedious task of antibody generation is entrusted to our skillful and experienced staff, which would ensure a timely delivery within 4 months; moreover, new antibodies are supplied at low cost, dramatically reducing your investment and risk; the use of innovative and advanced of technologies in our program would greatly increase your chance of success.  With this unique service, we believe that you would be able to advance your research at a much faster pace.

Pricing

Please contact us for an evaluation.